The type II interleukin-1 receptor (IL-1RII) of the bony fish gilthead seabream Sparus aurata is strongly induced after infection and tightly regulated at transcriptional and post-transcriptional levels

… Blots were probed with specific antibodies to V5, Xpress (Invitrogen) or the C-terminus of sbIL-1β (Pacific Immunology), and developed with enhanced chemiluminescence (ECL) reagents (Amersham Biosciences) according to the manufacturer’s protocol. 2.8. …
Gloria López-Castejóna, M. Pilar Sepulcrea, Francisco J. Rocaa, Barbara Castellanab, Josep V. Planasb, José Meseguera and Victoriano Mulero
Molecular Immunology, Volume 44, Issue 10, April 2007, Pages 2772-2780

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