The C-Terminal Domain of MinC Inhibits Assembly of the Z Ring in Escherichia coli
… performed with blots containing equal amounts of cell protein per lane, as described previously (1). Purified MinD protein from strain BL21(DE3) carrying pWM1682 (pET28a-his-minD) was used to raise rabbit anti-MinD polyclonal antiserum (Pacific Immunology, Ramona, CA). …
Daisuke Shiomi and William Margolin *
Journal of Bacteriology, January 2007, p. 236-243, Vol. 189, No. 1