Yes, Pacific Immunology® guarantees titers of at least 1:100,000 against peptide sequences that we recommend / approve, synthesize and conjugate. Titers are confirmed via ELISA at the 1st production bleed.
No, to avoid conflicts of interest, Pacific Immunology® focuses exclusively on the production of custom antisera for its clients.
Yes, clients maintain full IP ownership of custom antibodies.
Yes, Pacific Immunology® has for decades emphasized the importance of safeguarding clients’ confidential information.
Yes, Pacific Immunology specializes in the development of Rabbit Monoclonal antibodies with proprietary screening technologies to quickly and cost effectively deliver sequence-defined, recombinant monoclonal antibodies.
Pacific Immunology®'s ultra pure Freund's Adjuvants are comprised of premium components that enhance the immune response and significantly reduce immunization stresses for the animal. Please visit our AdjuLite™ Freund's Adjuvant page for additional details.
Yes, we offers discount for starting multiple projects at the same time.
Sodium Azide is a preservative that prevents bacteria from growing in the serum (bacteria can produce proteases that denature any proteins in the serum, including antibodies).
Serum is typically shipped via FedEx Overnight immediately after each bleed is taken.
A typical affinity purification of 25ml of serum will isolate approximately 2-3mg of peptide-specific antibody.
Antibody titers typically stabilize after the 1st production bleed with additional immunizations generally helping maintain (rather than increasing) antibody titers.
No, accelerated protocols are a marketing gimmick that delivers inferior, lower affinity antibodies.
New Zealand White Rabbits and Rhode Island Red Chickens
Yes, you will typically receive at least 30 mg of standalone peptide at the completion of the project.
We recommend sending at least 1-2 mg of the antigen for a rabbit or chicken project and 5 mg for a goat project. We recommend a concentration of at least .5 mg/ml if possible.
Please review our Antigen Preparation guidelines
No, insoluble proteins can be used for immunizations.
Yes, immunizing with gel bands can work well for generating polyclonal antisera against a purified protein. Please review our Antigen Preparation guidelines
Targeting full-length proteins allows for the development of antibodies against multiple epitopes throughout the protein, thereby increasing the probability of recognizing the native protein in the target assay. However, specificity is typically compromised for polyclonal projects. Expression of the protein may also be difficult or prohibitively expensive. Targeting peptide sequence can be a cost effective method to quickly isolate antibodies with high specificity to the target protein; but, binding capability with the native protein may be compromised in some assays.
Yes, our peptide chemists will analyze your protein sequence using an advanced suite of algorithms to recommend the most immunogenic sequences with a high probability of exposure.
The molecular weight of most standalone peptides is too low to generate an immune response in the animal.
An adjuvant is a substance that, when combined with the antigen, serves to enhance the immune response against the antigen. Freund's adjuvants are the preferred adjuvant of choice for use in antibody production, and Pacific Immunology® uses its own premium line of AdjuLite™ adjuvants for all immunizations. AdjuLite™ Complete Freund's Adjuvant is used only for the first immunization and contains mycobacteria as an additional stimulate for the host's immune system. AdjuLite™ Incomplete Freund's Adjuvant is used for all subsequent immunizations. Please visit AdjuLite™ Freund's Adjuvant for additional details.
Please review our Antibody Storage guidelines
Please review our Western Blot troubleshooting guidelines
Please review our Western Blot troubleshooting guidelines
The ELISA measures the peptide-specific immune response by comparing dilutions of the pre-immune serum and the first production bleed serum. A titer of 1:50,000 to 1:100,000 generally indicates a strong, specific immune response.
An IgG purification isolates all IgG in the serum, regardless of the specificity. This is most often used for projects targeting full-length protein where affinity purification may not be possible. Affinity purification, by contrast, is advantageous for isolating only those antibodies that recognize a small subset of epitopes (typically peptide targets), helping approach the specificity of monoclonal antibodies at a reduced cost.
Yes, the affinity column is included for purifying additional lots of serum.