Introduction
The following citations highlight custom antibodies that have been developed by Pacific Immunology® and successfully used by researchers to publish their research in peer-reviewed journals.
RivR is a negative regulator of virulence factor expression in the group A Streptococcus
… 186 187 Western blot analysis. Protein samples were separated on 12% Tris-HCl gels. An 188 affinity purified rabbit-anti-GRAB antibody was generated by Pacific Immunology Corp 189 from rabbits that were co-immunized with two peptide fragments of GRAB (Figure S1), 190 …
J Treviño, Z Liu, TN Cao, E Ramirez-Peña, P Sumby –
Infection and Immunity, 2012
Transgenic zebrafish model of the C43G human insulin gene mutation
… Louis, MO, USA; 1:200), monoclonal mouse anti-KDEL (Abcam, Cambridge, MA, USA; 1:200), monoclonal rabbit anti-BiP (Cell Signaling, Danvers, MA, USA; 1:200) and monospecific rabbit anti-zebrafish C-peptide (Pacific Immunology, Ramona, CA, USA; 1:500). …
SC Eames, MD Kinkel, S Rajan, VE Prince, LH Philipson
Journal of Diabetes Investigation 2012
Regulation of lipid synthesis by liver X receptor α and sterol regulatory element-binding protein 1 in mammary epithelial cells
… Western blot analysis of total ACC1 (rabbit anti-human ACC polyclonal antibody, 3662; Cell Signaling Technology Inc., Danvers, MA), SCD1 (custom synthesized rabbit anti-bovine antibody; Pacific Immunology Corp., Ramona, CA), FAS (rabbit anti-human FAS monoclonal …
C Oppi-Williams, JK Suagee, BA Corl
Journal of Dairy Science, 2012
Leptospiral Outer Membrane Protein Microarray, a Novel Approach to Identification of Host Ligand-Binding Proteins
… gel. New Zealand White rabbits were immunized (Pacific Immunology, Ramona, CA) with 0.25 mg of gel-purified recombinant protein four times over a 9-week period, and serum was collected 1 week after the final injection. …
M Pinne, J Matsunaga, DA Haake
Journal of Bacteriology, 2012
VraT/YvqF is required for methicillin resistance and activation of the VraSR regulon in Staphylococcus aureus
… Bind Kit (Novagen) as recommended by the manufacturer. Antiserum was collected from 169 rabbits after 4 injections with VraR (Pacific Immunology). The specificity of the antiserum for 170 VraR is demonstrated in Results. …
S Boyle-Vavra, S Yin, DS Jo, C Montgomery, RS Daum –
Antimicrobial Agents and Chemotherapy, 2012