Introduction
The following citations highlight custom antibodies that have been developed by Pacific Immunology® and successfully used by researchers to publish their research in peer-reviewed journals.
Upregulation of sterol C14-demethylase expression in Trypanosoma cruzi treated with sterol biosynthesis inhibitors
… 2.6. Peptides and antibodies. Synthetic peptides and monospecific antibodies were made as a service by Pacific Immunology (Ramona, CA). Detailed information about their methods can be obtained at Pacific Immunology (website: http://www.pacificimmunology.com/). …
Eleanor G. Hankins, John R. Gillespie, Kendra Aikenhead and Frederick S. Buckner*
Molecular and Biochemical Parasitology / Volume 144, Issue 1, November 2005, Pages 68-75
Novel Lymphotoxin Alpha (LT) Knockout Mice with Unperturbed Tumor Necrosis Factor Expression: Reassessing LT Biological Functions
… of body weight intravenously and were observed for 12 h. For ovalbumin (OVA)/complete Freund’s adjuvant (CFA) immunization, mice were injected intraperitoneally with 50 μg OVA in 25 μl of saline emulsified 1:1 in AdjuLite Freund’s adjuvant (Pacific Immunology, Ramona, CA …
Dmitry J. Liepinsh,1,2 Sergei I. Grivennikov,2,6 Kimberly D. Klarmann,1,3 Maria A. Lagarkova,6 Marina S. Drutskaya,3,6 Stephen J. Lockett,4 Lino Tessarollo,5 Matthew McAuliffe,7 Jonathan R. Keller,1,3 Dmitry V. Kuprash,2,6 and Sergei A. Nedospasov6*
Molecular and Cellular Biology, June 2006, p. 4214-4225, Vol. 26, No. 11
Characterization of the Transcriptional Activators SalA and SyrF, Which Are Required for Syringomycin and Syringopeptin Production by Pseudomonas syringae pv. Syringae
… Polyclonal antiserum with antibodies recognizing SyrF-MBP was commercially produced by immunization of rabbits with purified SyrF protein (Pacific Immunology Corp., CA). Western blots were performed as described above. …
Nian Wang, Shi-En Lu, Angela R. Records, and Dennis C. Gross*
Journal of Bacteriology, May 2006, p. 3290-3298, Vol. 188, No. 9
Regulation of diacylglycerol acyltransferase in developing seeds of castor
… Antibody production. Antibodies for RcDGAT were raised in two rabbits immunized with the peptide (CVLLYYHDLM- NRDGN), which corresponds to the C terminus of the native protein (Pacific Immunology Corp., Ramona, CA). …
Xiaohua He, Grace Q. Chen, Jiann-Tsyh Lin and Thomas A. McKeon*
Lipids, Volume 39, Number 9 / September, 2004
The C-Terminal Domain of MinC Inhibits Assembly of the Z Ring in Escherichia coli
… performed with blots containing equal amounts of cell protein per lane, as described previously (1). Purified MinD protein from strain BL21(DE3) carrying pWM1682 (pET28a-his-minD) was used to raise rabbit anti-MinD polyclonal antiserum (Pacific Immunology, Ramona, CA). …
Daisuke Shiomi and William Margolin *
Journal of Bacteriology, January 2007, p. 236-243, Vol. 189, No. 1