Introduction
The following citations highlight custom antibodies that have been developed by Pacific Immunology® and successfully used by researchers to publish their research in peer-reviewed journals.
Phosphorylation of the alternative mRNA SPF45 by Clk1 regulates its splice site utilization, cell migration and invasion
…Polyclonal anti-SPF45 antibody was generated to recombinant His-SPF45 protein in rabbits (Pacific Immunology, San Diego, CA). Secondary antibodies conjugated to horseradish peroxidase were followed by enhanced chemiluminescence. Results were confirmed by at least three independent experiments…
Yuying Liu1, LaShardai Conaway1, Jennifer Rutherford Bethard1, Adnan M. Al-Ayoubi1, Amber Thompson Bradley1, Hui Zheng1, Scott A. Weed2 and Scott T. Eblen1,3,*
Nucleic Acids Research 2013 doi: 10.1093/nar/gkt170
Visit Citation Link
Calcitonin receptor 1 (AedaeGPCRCAL1) hindgut expression and direct role in myotropic action in females of the mosquito Aedes aegypti (L.)
…The blot was incubated with rabbit anti-AedaeGPCRCAL1 (antigen sequence: C-RGYAGTPEDTIE corresponding to the receptor second extracellular loop) affinity purified antibodies (1:500 dilution; 1 mg/ml final concentration) (Pacific Immunology, Ramona, CA, USA) on a shaker at 4C overnight…
Hyeogsun Kwon, Patricia V. Pietrantonio
Insect Biochemistry and Molecular Biology Volume 43, Issue 7, July 2013, Pages 588–593
Visit Citation Link
EscE and EscG are co-chaperones for the type III needle protein EscF of enteropathogenic E. coli
…Type III secretion systems (T3SS) are central virulence mechanisms used by a variety of Gram-negative bacteria to inject effector proteins into host cells. The needle polymer is an essential part of the T3SS that provides the effector proteins a continuous channel into the host cytoplasm…
Neta Sal-Man1, Dheva Setiaputra1, Roland Scholz1, Wanyin Deng1, Angel C.Y. Yu2,4, Natalie C.J. Strynadka2,4 and B. Brett Finlay1,2,3,*
Journal of Bacteriology March 2013, doi: 10.1128/JB.00118-13
Visit Citation Link
Identification and characterization of a periplasmic trilactone esterase, Cee, revealed unique features of ferric enterobactin acquisition in Campylobacter
… analysis and biochemical assay strongly suggested that Cee is the sole trilactone esterase in C. jejuni. Thin-layer chromatography and HPLC analyses showed high efficiency of the purified Cee to hydrolyse Ent. Three Cee homologues previously characterized from other bacteria (IroE, IroD and Fes) were also purified …
Ximin Zeng1, Yiming Mo1, Fuzhou Xu1,2, Jun Lin
Molecular Microbiology DOI: 10.1111/mmi.12118
Visit Citation Link
N-terminal phosphorylation sites of herpes simplex virus type 1 ICP0 differentially regulate its activities and enhance viral replication
… protein G Dynabeads (Invitrogen, cat. no.77149610) were incubated, after aspirating the 228 beads buffer, with 200 µL of PBS with 0.05% Tween-20 and 4 µL of an ICP0 rabbit 229 polyclonal antibody (Pacific Immunology) by rotating for 1 h at room temperature. Beads 230 …
Heba H. Mostafa, Thornton W. Thompson and David J. Davido*
Journal of Virology, 2012 doi: 10.1128/JVI.02588-12
Visit Citation Link