Introduction
The following citations highlight custom antibodies that have been developed by Pacific Immunology® and successfully used by researchers to publish their research in peer-reviewed journals.
A detergent-free strategy for the reconstitution of active enzyme complexes from native biological membranes into nanoscale discs
…For immunodetection of mitochondrial proteins, samples were resuspended in SIB and solubilized with DDM or SMA as indicated. Following transfer onto nitrocellulose membranes, Western blotting was performed with primary antibodies against the Sdh3 subunit of respiratory Complex II (Pacific Immunology) or against the Tim50 subunit of the TIM23 Complex (a gift from Dr. Dejana Mokranjac) and Amersham ECL Plex Cy5-conjugated secondary antibodies.
Ashley R Long1, Catherine C O’Brien1, Ketan Malhotra1, Christine T Schwall1, Arlene D Albert1, Anthony Watts2 and Nathan N Alder
BMC Biotechnology 2013, 13:41 doi:10.1186/1472-6750-13-41
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Mutualistic Polydnaviruses Share Essential Replication Gene Functions with Pathogenic Ancestors
…A polyclonal antibody was then produced by a commercial service (Pacific Immunology) which generated antisera in rabbits by immunizing with ca. 500 µg of affinity purified truncated LEF-9. Antiserum was then purified by nitrocellulose-based immunoaffinity chromatography as previously outlined [65], [66]. The resulting antibody was then used in immunoblotting experiments with control and lef-9 knockdown wasps…
Gaelen R. Burke mail, Sarah A. Thomas, Jai H. Eum, Michael R. Strand
PLoS Pathog 9(5): e1003348. doi:10.1371/journal.ppat.1003348 May 9, 2013
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Structural And Functional Characterization Of Mulitdrug Resistance Transporter And Regulator
…Drug resistant bacteria pathogen poses a severe threat to human health. Bacterial drug efflux pumps are transporter proteins involved in the export of antibiotics out of cells. Efflux by transporters is one of the major drug resistant mechanisms… The custom polyclonal rabbit anti-AcrA and TolC were ordered from Pacific Immunology (Ramona CA)
Yu, Linliang
University of Kentucky (2013). Theses and Dissertations–Chemistry. Paper 19
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Evolution of the Human Gastrokine Locus and Confounding Factors Regarding the Pseudogenicity of Gkn3
…In a screen for genes expressed specifically in gastric mucous neck cells, we identified GKN3, the recently discovered third member of the gastrokine family. We present confirmatory mouse data and novel porcine data showing that mouse GKN3 expression is confined to mucous cells of the corpus neck and antrum base and is prominently expressed in metaplastic lesions…
Jessica H. Geahlen1, Carlo Lapid1, Kaisa Thorell2, Igor Nikolskiy1, Won Jae Huh1, Edward L Oates1, Jochen K.M. Lennerz3, Xiaolin Tian1, Victoria G. Weis4, Shradha S Khurana1, Samuel B Lundin2, Alan R Templeton1, and Jason C Mills
Physiological Genomics May 28, 2013, doi: 10.1152/physiolgenomics.00169.2012
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Staphopains modulate Staphylococcus aureus biofilm integrity
…Staphylococcus aureus is a known cause of chronic biofilm infections that can reside on medical implants or host tissue. Recent studies have demonstrated an important role for proteinaceous material in the biofilm structure. The S. aureus genome encodes many secreted proteases, and there is growing evidence that these enzymes have self-cleavage properties that alter biofilm integrity. …sent for the production of chicken antibodies to ScpA (Pacific Immunology)…
Joe M. Mootz1, Cheryl L. Malone1, Lindsey N. Shaw2 and Alexander R. Horswill
ASM Infection and Immunity 24 June 2013, doi: 10.1128/IAI.00377-13
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