Introduction
The following citations highlight custom antibodies that have been developed by Pacific Immunology® and successfully used by researchers to publish their research in peer-reviewed journals.
Membrane topology of human AGPAT3 (LPAAT3)
… Peptide antibody for AGPAT3/LPAAT3 was designed and generated by Pacific Immunology (Ramona, CA) and characterized as described [7]. Rabbit anti-GFP was kindly provided by Anthony Bretscher (Weill Institute of Cell and Molecular Biology, Cornell University). …
John A. Schmidt, Griselda Metta Yvone, William J. Brown
Biochemical and Biophysical Research Communications Volume 397, Issue 4, 9 July 2010, Pages 661–667
Visit Citation Link
Immunolocalization of the short neuropeptide F receptor in queen brains and ovaries of the red imported fire ant (Solenopsis invicta Buren)
… After purification, the specificity of the antibodies was verified by ELISA (tested by Pacific Immunology, CA) and western blot. Membrane preparations and western blot analysis Membrane preparations for western blot analyses were performed as …
Hsiao-Ling Lu and Patricia V Pietrantonio
BMC Neuroscience 2011, 12:57 doi:10.1186/1471-2202-12-57
Visit Citation Link
Deconstructing honeybee vitellogenin: novel 40 kDa fragment assigned to its N terminus
… After 3×15 min washes with PBS-T, sections were incubated overnight at 4°C with a polyclonal (rabbit) anti-vitellogenin antibody at 1:500 (raised against 180 kDa honeybee vitellogenin; Pacific Immunology, Ramona, CA, USA); specificity had been tested and confirmed …
Heli Havukainen, Øyvind Halskau, Lars Skjaerven, Bente Smedal1 and Gro V. Amdam
doi: 10.1242/jeb.048314 February 15, 2011 J Exp Biol 214, 582-592.
Visit Citation Link
Rot and SaeRS cooperate to activate expression of the staphylococcal superantigen-like exoproteins
… Generation of anti-Rot polyclonal antibody.The rot gene was amplified, cloned, expressed, and purified as described above. The Rot polyclonal antibody was generated by Pacific Immunology by immunizing rabbits with the purified protein. …
Meredith A. Benson, Sarit Lilo, Tyler Nygaard, Jovanka M. Voyich and Victor J. Torres
Published ahead of print 8 June 2012, doi: 10.1128/JB.00706-12 JB.00706-12
Visit Citation Link
Characterization of acyl carrier protein and LytB in Babesia bovis apicoplast.
… Antibody production of rmACP68-148 was 19 subsequently generated (Pacific Immunology Corp., CA), affinity-purified using 20 HiTrapâ„¢ Protein A HP Columns (GE Healthcare) and validated by western blot 21 analysis using B. bovis infected erythrocytes, uninfected …
Marina C. Caballero,a Monica J. Pedroni,b Guy H. Palmer,a Carlos E. Suarez,ab Christine Davitt,c and Audrey O.T. Laua
Mol Biochem Parasitol. 2012 February; 181(2): 125–133. doi: 10.1016/j.molbiopara.2011.10.009
Visit Citation Link