Introduction

The following citations highlight custom antibodies that have been developed by Pacific Immunology® and successfully used by researchers to publish their research in peer-reviewed journals.


Mismatch repair in Trypanosoma brucei: Heterologous expression of MSH2 from Trypanosoma cruzi provides new insights into the response to oxidative damage

… Proteins were separated in 10% polyacrylamide gels containing 0.1% SDS using standard methods for SDS-PAGE, transferred to nitrocellulose membranes and incubated with primary (polyclonal anti-TbMSH2 diluted 1:600, Pacific Immunology; monoclonal anti-alpha tubulin …
A Machado-Silva, SMR Teixeira, GR Franco… –
Gene, 2008 – Elsevier

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A homologous expression system for obtaining engineered cytochrome ba3 from Thermus thermophilus HB8

… Louis, MO). Polyclonal antibodies against purified, soluble Cu A protein (subunit II of ba 3 , [45]) were obtained from Pacific Immunology (Ramona CA). Cytochrome rsC 552 (native-like, Thermus cytochrome c 552 ) was obtained by the method of Fee et al. [44]. …
Y Chen, L Hunsicker-Wang, RL Pacoma, E Luna… –
Protein expression and …, 2005 – Elsevier

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The rapid activation of N-Ras by [alpha]-thrombin in fibroblasts is mediated by the specific G-protein G [alpha] i2-G [beta] 1-G [gamma] 5 and occurs in lipid rafts

… 371751 and 371754), α-Gα 13 (Calbiochem, 371729), α-Gβ 1 (Calbiochem, 371813), α-Gβ 3 (Calbiochem, 371819), α-Gβ 4 (Santa Cruz, sc-382), α-Gβ 5 (Calbiochem, 371819), α-Gγ 5 (Santa Cruz, sc-376), α-Gγ 7 (Santa Cruz, sc-377), α-Gγ 11 (Pacific Immunology), α-GRF2 …
Nathan H. Lentsb, , , Virginia Irintchevaa, Reema Goela, Leroy W. Wheelera, Joseph J. Baldassare
Cellular signalling, 2009 – Elsevier

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The Iron-Responsive Fur/RyhB Regulatory Cascade Modulates the Shigella Outer Membrane Protease IcsP

… cell density. Western blot analysis was performed with an affinity-purified IcsP rabbit antiserum (Pacific Immunology) and detected by chemiluminescence using a UVP BioSpectrum 410 imaging system. Integrated densitometry …
Lia A. A. Africa1,‡§, Erin R. Murphy2,‡, Nicholas R. Egan1, Amanda F. Wigley1 and Helen J. Wing1,*
Infection and Immunology, 2011 – Am Soc Microbiol
http://iai.asm.org/content/79/11/4543.short

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Molecular, antigenic, and functional characteristics of ferric enterobactin receptor CfrA in Campylobacter jejuni

… Approximately 2 mg of highly purified rCfrA obtained from JL275 was used for production of rabbit polyclonal antisera against CfrA. The rabbit CfrA polyclonal antisera were prepared by Pacific Immunology Corp. (Ramona, CA). …
X Zeng, F Xu, J Lin –
Infection and immunity, 2009 – Am Soc Microbiol

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