Introduction
The following citations highlight custom antibodies that have been developed by Pacific Immunology® and successfully used by researchers to publish their research in peer-reviewed journals.
Estrogen Receptor-α Variant, ER-α36, is Involved in Tamoxifen Resistance and Estrogen Hypersensitivity
…Antiestrogens such as tamoxifen provided a successful treatment for ER-positive breast cancer for the past two decades. However, most breast tumors are eventually resistant to tamoxifen therapy. The molecular mechanisms underlying tamoxifen resistance have not been well established. Recently, we reported that breast cancer patients with tumors expressing high concentrations of ER-α36…
XianTian Zhang and Zhao-Yi Wang
Endocrinology June 1, 2013 vol. 154 no. 6 1990-1998
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Activated Cdc42 kinase regulates Dock localization in male germ cells during Drosophila spermatogenesis
…Deregulation of the non-receptor tyrosine kinase ACK1 (Activated Cdc42-associated kinase) correlates with poor prognosis in cancers and has been implicated in promoting metastasis. To further understand its in vivo function, we have characterized the developmental defects of a null mutation in Drosophila Ack, which bears a high degree of sequence similarity to…
Abbas M. Abdallah, Xin Zhou, Christine Kim, Kushani K. Shah, Christopher Hogden, Jessica A. Schoenherr, James C. Clemens, Henry C. Chang
Developmental Biology Volume 378, Issue 2, 15 June 2013, Pages 141–153
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Cholesterol efflux in megakaryocyte progenitors suppresses platelet production and thrombocytosis
…The rabbit antibody to ABCG4 was custom made by Pacific Immunology (CA, USA) against a synthetic ABCG4 peptide (KKVENHITEAQRFSHLPKR). Monospecific anti-peptide antibodies werepurified using a peptide-affinity column. The specificity of the antibody forABCG4 protein was assessed by immunofluorescence microscopy, which showed specific immunofluorescence signals in HEK293 cells expressing ABCG4 but notHEK293 cells transfected with mock vectors…
Andrew J Murphy, Nora Bijl, Laurent Yvan-Charvet, Carrie B Welch, Neha Bhagwat, Adili Reheman, Yiming Wang, James A Shaw, Ross L Levine, Heyu Ni, Alan R Tall & Nan Wang
Nature Medicine 19, 586–594 (2013) doi:10.1038/nm.3150
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Infusion of imaging and therapeutic molecules into the plant virus-based carrier cowpea mosaic virus: Cargo-loading and delivery
…This work is focused on the development of a plant virus-based carrier system for cargo delivery, specifically 30 nm-sized cowpea mosaic virus (CPMV). Whereas previous reports described the engineering of CPMV through genetic or chemical modification, we report a non-covalent infusion technique that facilitates efficient cargo loading…
Ibrahim Yildiz, Karin L. Lee, Kevin Chen, Sourabh Shukla, Nicole F. Steinmetz
Journal of Controlled Release 9 May 2013
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Characterization of Neospora caninum macrophage migration inhibitory factor
…The present study is the first characterization of Neospora caninum macrophage migration inhibitory factor (NcMIF). BLAST-N analysis of NcMIF revealed high similarity (87%) to the Toxoplasma gondii MIF. NcMIF was cloned and expressed in Escherichia coli in 3 forms…
Guanggang Qua, Raymond Fetterer, Mark Jenkins, Lin Leng, Zhiqiang Shen, Charles Murphyd, Wenyu Hane, Richard Bucala, Wenbin Tuo
Experimental Parasitology 10 July 2013
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