Inhibitor of Nrf2 (INrf2 or Keap1) Protein Degrades Bcl-xL via Phosphoglycerate Mutase 5 and Controls Cellular Apoptosis
… catalog no. ALX-201073). We generated and purified bacterial PGAM5L-His-tagged protein. The polyclonal antibodies against the PGAM5L form were generated in rabbits (Pacific Immunology) and purified. The membranes …
SK Niture, AK Jaiswal –
Journal of Biological Chemistry, 2011 – ASBMB
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Mismatch repair in Trypanosoma brucei: Heterologous expression of MSH2 from Trypanosoma cruzi provides new insights into the response to oxidative damage
… Proteins were separated in 10% polyacrylamide gels containing 0.1% SDS using standard methods for SDS-PAGE, transferred to nitrocellulose membranes and incubated with primary (polyclonal anti-TbMSH2 diluted 1:600, Pacific Immunology; monoclonal anti-alpha tubulin …
A Machado-Silva, SMR Teixeira, GR Franco… –
Gene, 2008 – Elsevier
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A homologous expression system for obtaining engineered cytochrome ba3 from Thermus thermophilus HB8
… Louis, MO). Polyclonal antibodies against purified, soluble Cu A protein (subunit II of ba 3 , [45]) were obtained from Pacific Immunology (Ramona CA). Cytochrome rsC 552 (native-like, Thermus cytochrome c 552 ) was obtained by the method of Fee et al. [44]. …
Y Chen, L Hunsicker-Wang, RL Pacoma, E Luna… –
Protein expression and …, 2005 – Elsevier
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Molecular, antigenic, and functional characteristics of ferric enterobactin receptor CfrA in Campylobacter jejuni
… Approximately 2 mg of highly purified rCfrA obtained from JL275 was used for production of rabbit polyclonal antisera against CfrA. The rabbit CfrA polyclonal antisera were prepared by Pacific Immunology Corp. (Ramona, CA). …
X Zeng, F Xu, J Lin –
Infection and immunity, 2009 – Am Soc Microbiol
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The tubulin-like RepX protein encoded by the pXO1 plasmid forms polymers in vivo in Bacillus anthracis
… sulfate-polyacrylamide gel electrophoresis. The purified protein was used to raise anti-RepX polyclonal antibodies in rabbits, and the antibodies were affinity purified (Pacific Immunology, Ramona, CA). For immunoblot analysis …
P Akhtar, SP Anand, SC Watkins… –
Journal of bacteriology, 2009 – Am Soc Microbiol
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