Sex reversal of the amphibian, Xenopus tropicalis, following larval exposure to an aromatase inhibitor
… Vitellogenin was purified by ion-exchange chromatography from the collected plasma at the Interdisciplinary Center for Biotechnology Research at the University of Florida (Gainesville, FL). Rabbit polyclonal antibodies were prepared by Pacific Immunology Corp (Ramona, CA). …
AW Olmstead, PA Kosian, JJ Korte, GW Holcombe… –
Aquatic Toxicology, 2009 – Elsevier
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Cytolocalization of the class V chitin synthase in the yeast, hyphal and sclerotic morphotypes of Wangiella (Exophiala) dermatitidis
… The WdMyo12p (2 mg) in Freund’s incomplete adjuvant was used to immunize the New Zealand White Rabbit (Pacific Immunology, CA, USA) from which blood samples had been previously obtained for use as preimmune serum. …
D Abramczyk, C Park, PJ Szaniszlo –
Fungal Genetics and Biology, 2009 – Elsevier
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Reproductive maturation of the tropical clawed frog: Xenopus tropicalis
… Florida (Gainesville, FL). The purified vitellogenin was at a concentration of 1.2 mg/ml and stored in aliquots at −80 °C. Vitellogenin antiserum was prepared in rabbits by Pacific Immunology Corp (Ramona, CA). Prior to use, the …
AW Olmstead, JJ Korte, KK Woodis, BA Bennett… –
General and …, 2009 – Elsevier
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Amblyomma americanum (L.)(Acari: Ixodidae) tick salivary gland serine protease inhibitor (serpin) 6 is secreted into tick saliva during tick feeding
… Production of rabbit polyclonal antibodies to rAamS6. The production of rabbit polyclonal antibodies to the rAamS6 protein fused with a 6-His tag at the amino-terminal region was subcontracted to a fee-for-service company (Pacific Immunology, Ramona, CA, USA). …
KC Chalaire, TK Kim, H Garcia-Rodriguez… –
Journal of …, 2011 – jeb.biologists.org
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Sinorhizobium meliloti ExoR is the target of periplasmic proteolysis
… Purification of the His-tagged ExoR protein was confirmed using Coomassie blue-stained SDS-polyacrylamide gels and sent to the Pacific Immunology Company (Ramona, CA) to raise ExoR-specific polyclonal antibodies in rabbits. …
HY Lu, L Luo, MH Yang, HP Cheng –
Journal of Bacteriology, 2012 – Am Soc Microbiol
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