Porcine IgE in the context of experimental food allergy: Purification and isotype-specific antibodies
… Two New Zealand White rabbits were immunized by Pacific Immunology Corp., Ramona, CA, USA (approved by NIH OLAW Assurance # A4182-01 and USDA Registration # 93-R-283, USA) using the gel slice-derived pig IgE heavy chain (2 mg total). …
P Rupa, K Hamilton, M Cirinna, BN Wilkie –
Veterinary immunology and …, 2008 – Elsevier
Disruption of a ciliary B9 protein complex causes Meckel syndrome
… Smo antibodies were generated by injection of a Smo-MBP fusion protein comprising the C-terminal tail of mouse Smo into rabbits (Pacific Immunology) and affinity purified with recombinant GST-Smo C terminus. qRT-PCR. …
WE Dowdle, JF Robinson, A Kneist… –
The American Journal of …, 2011 – Elsevier
Brood pheromone suppresses physiology of extreme longevity in honeybees (Apis mellifera)
… After 3×15 min washes with PBS-T, sections were incubated overnight at 4°C with a polyclonal (rabbit) anti-vitellogenin antibody at 1:500 (raised against 180 kDa honeybee vitellogenin, Pacific Immunology, Ramona, CA, USA); specificity was tested and confirmed previously …
B Smedal, M Brynem, CD Kreibich… –
Journal of Experimental …, 2009 – jeb.biologists.org
Identification and Characterization of a New Ferric Enterobactin Receptor, CfrB, in Campylobacter
… sequence of cfrB were detected. Approximately 3 mg of highly purified rCfrB was used for the production of rabbit polyclonal antisera against CfrB by Pacific Immunology Corp. (Ramona, CA). Pre- and postimmune serum samples …
F Xu, X Zeng, RD Haigh, JM Ketley, J Lin –
Journal of bacteriology, 2010 – Am Soc Microbiol
A novel calmodulin-like protein from the liver fluke, Fasciola hepatica
… Aliquots (1 ml, 1 mg.ml −1 ) of purified, recombinant FhCaM2 and FhCaM3 were sent to Pacific Immunology (Ramona, CA, USA) for antiserum production in rabbits and these antisera were used as primary antibodies against FhCaM2 and FhCaM3. 2.8. ELISAs. …
SL Russell, NV McFerran, CM Moore, Y Tsang, P Glass… –
Biochimie, 2012 – Elsevier