Structural characterization of CFA/III and longus type IVB pili from enterotoxigenic Escherichia coli
… Cells were imaged using an FEI F20 Supertwin electron microscope. Production of anti-CofA antibodies.The ΔN-CofA protein used for crystallization was used as an antigen for polyclonal rabbit anti-CofA antibody production (Pacific Immunology). …
S Kolappan, J Roos, ASW Yuen, OM Pierce… –
Journal of …, 2012 – Am Soc Microbiol
Intracellular assembly of cyanophage Syn5 proceeds through a scaffold-containing procapsid
… A mixture of primary rabbit antibodies—anti-Syn5 (Covance), anti-gp53 (Strategic Diagnostics), anti-gp54 (Pacific Immunology), and anti-gp58 (Strategic Diagnostics), all diluted 1:1,000—was used for the membrane probing whose results are shown in Fig. 3b. …
DA Raytcheva, C Haase-Pettingell, JM Piret… –
Journal of …, 2011 – Am Soc Microbiol
Molecular cloning and functional characterization of the avian macrophage migration inhibitory factor (MIF)
… 2.6. Production of rabbit anti-rChMIF polyclonal antisera and Western blot analysis. Antisera production was performed by Pacific Immunology (Romona, CA) by immunizing rabbits with rChMIF purified by SEC-HPLC as described in Section 2.5. …
Sungwon Kima, Kate B. Miskab, Mark C. Jenkinsb, Ray H. Fettererb, Chasity M. Coxa, Lindsay H. Stuarda, Rami A. Dalloula
Developmental & Comparative Immunology Volume 34, Issue 9, September 2010, Pages 1021–1032
CPMV-DOX Delivers
… using 5% (v/v) GS for 45 min. CPMV was stained using specific antibodies: cells were incubated with anti-CPMV antibodies (rabbit IgG, Pacific Immunology) at 1:200 dilution for 60 min at room temperature. Endolysosomes were stained using a mouse anti-human …
DJ Evans, A Aljabali, S Shukla… –
Molecular …, 2012 – ACS Publications
Treponema denticola Superoxide Reductase: In Vivo Role, In Vitro Reactivities and a Novel [Fe (Cys) 4] Site
… o C. Td SOR Antibodies and Western Blotting. Antisera against purified recombinant Td SOR (without the His tag) were raised in rabbits (Pacific Immunology Corp). Western blotting using the rabbit antiserum followed a standard procedure (28). Iron/Protein Mol Ratios. …
JD Caranto, LL Gebhardt, CE MacGowan… –
Biochemistry, 2012 – ACS Publications