Bacterial Pili exploit integrin machinery to promote immune activation and efficient blood-brain barrier penetration
… rabbits with PilA–(His)6 protein preparation mixed with Freund’s complete adjuvant (Pacific Immunology)….
Anirban Banerjee,1 Brandon J. Kim,1,4 Ellese M. Carmona,1,4 Andrew S. Cutting,1 Michael A. Gurney,1 Chris Carlos,1 Ralph Feuer,1 Nemani V. Prasadarao,2 and Kelly S. Dorana,1,3
Nat Commun. 2011 September 6; 2: 462. doi: 10.1038/ncomms1474
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A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition
… Smo ( Pacific Immunology) and affinity purified using a recombinant …
Francesc R Garcia-Gonzalo, Kevin C Corbit, María Salomé Sirerol-Piquer, Gokul Ramaswami, Edgar A Otto, Thomas R Noriega, Allen D Seol, Jon F Robinson, Christopher L Bennett, Dragana J Josifova, José Manuel García-Verdugo, Nicholas Katsanis, Friedhelm Hildebrandt & Jeremy F Reiter
Nature Genetics 43, 776–784 (2011) doi:10.1038/ng.891
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Cdc14 Phosphatases preferentially dephosphorylate a subset of cyclin-dependent kinase (Cdk) sites containing phosphoserine
… Extracts were then separated by SDS-PAGE on a 10% gel and analyzed by immunoblotting. Acm1 and Acm1-S4T were detected with an affinity-purified polyclonal antibody directed against recombinant GST-Acm1 (Pacific Immunology, 0.04 μg/ml working concentration). …
SC Bremmer, H Hall, JS Martinez, CL Eissler… –
Journal of Biological …, 2012 – ASBMB
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Association and evidence for linked recognition of type IV secretion system proteins VirB9-1, VirB9-2, and VirB10 in Anaplasma marginale
… diluted 1:500. A negative control rabbit antiserum was made by Pacific Immunology, using Freund complete and incomplete adjuvants and a fragment of a B. bovis nuclear-encoded protein, acyl carrier protein (ACP). The ACP …
K Morse, J Norimine, GH Palmer, EL Sutten… –
Infection and …, 2012 – Am Soc Microbiol
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A Drosophila Model of Spinal Muscular Atrophy Uncouples snRNP Biogenesis Functions of Survival Motor Neuron from Locomotion and Viability Defects
… Western analysis was performed using standard protocols. Rabbit anti-dSMN serum was generated by injecting rabbits with purified full-length dSMN protein (Pacific Immunology Corp., Ramona, CA, USA) and was subsequently affinity purified. …
K Praveen, Y Wen, AG Matera –
Cell Reports, 2012 – Elsevier
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