Evolution of the Human Gastrokine Locus and Confounding Factors Regarding the Pseudogenicity of Gkn3
…In a screen for genes expressed specifically in gastric mucous neck cells, we identified GKN3, the recently discovered third member of the gastrokine family. We present confirmatory mouse data and novel porcine data showing that mouse GKN3 expression is confined to mucous cells of the corpus neck and antrum base and is prominently expressed in metaplastic lesions…
Jessica H. Geahlen1, Carlo Lapid1, Kaisa Thorell2, Igor Nikolskiy1, Won Jae Huh1, Edward L Oates1, Jochen K.M. Lennerz3, Xiaolin Tian1, Victoria G. Weis4, Shradha S Khurana1, Samuel B Lundin2, Alan R Templeton1, and Jason C Mills
Physiological Genomics May 28, 2013, doi: 10.1152/physiolgenomics.00169.2012
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Staphopains modulate Staphylococcus aureus biofilm integrity
…Staphylococcus aureus is a known cause of chronic biofilm infections that can reside on medical implants or host tissue. Recent studies have demonstrated an important role for proteinaceous material in the biofilm structure. The S. aureus genome encodes many secreted proteases, and there is growing evidence that these enzymes have self-cleavage properties that alter biofilm integrity. …sent for the production of chicken antibodies to ScpA (Pacific Immunology)…
Joe M. Mootz1, Cheryl L. Malone1, Lindsey N. Shaw2 and Alexander R. Horswill
ASM Infection and Immunity 24 June 2013, doi: 10.1128/IAI.00377-13
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Phosphorylation of the alternative mRNA SPF45 by Clk1 regulates its splice site utilization, cell migration and invasion
…Polyclonal anti-SPF45 antibody was generated to recombinant His-SPF45 protein in rabbits (Pacific Immunology, San Diego, CA). Secondary antibodies conjugated to horseradish peroxidase were followed by enhanced chemiluminescence. Results were confirmed by at least three independent experiments…
Yuying Liu1, LaShardai Conaway1, Jennifer Rutherford Bethard1, Adnan M. Al-Ayoubi1, Amber Thompson Bradley1, Hui Zheng1, Scott A. Weed2 and Scott T. Eblen1,3,*
Nucleic Acids Research 2013 doi: 10.1093/nar/gkt170
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Identification and characterization of a periplasmic trilactone esterase, Cee, revealed unique features of ferric enterobactin acquisition in Campylobacter
… analysis and biochemical assay strongly suggested that Cee is the sole trilactone esterase in C. jejuni. Thin-layer chromatography and HPLC analyses showed high efficiency of the purified Cee to hydrolyse Ent. Three Cee homologues previously characterized from other bacteria (IroE, IroD and Fes) were also purified …
Ximin Zeng1, Yiming Mo1, Fuzhou Xu1,2, Jun Lin
Molecular Microbiology DOI: 10.1111/mmi.12118
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N-terminal phosphorylation sites of herpes simplex virus type 1 ICP0 differentially regulate its activities and enhance viral replication
… protein G Dynabeads (Invitrogen, cat. no.77149610) were incubated, after aspirating the 228 beads buffer, with 200 µL of PBS with 0.05% Tween-20 and 4 µL of an ICP0 rabbit 229 polyclonal antibody (Pacific Immunology) by rotating for 1 h at room temperature. Beads 230 …
Heba H. Mostafa, Thornton W. Thompson and David J. Davido*
Journal of Virology, 2012 doi: 10.1128/JVI.02588-12
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