Discovery and characterization of a second mammalian thiol dioxygenase: Cysteamine dioxygenase
… Although this was not a problem for the production of antibody, it precluded the use of the fusion protein for enzyme activity assays. Purified protein was sent to Pacific Immunology, Inc. (Ramona, CA) for polyclonal antibody production in New Zealand White rabbits. …
John E. Dominy . Jr, Chad R. Simmons, Lawrence L. Hirschberger, Jesse Hwang, Relicardo M. Coloso, and Martha H. Stipanuk
J. Biol. Chem, 10.1074/jbc.M703089200
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Characterization of the Transcriptional Activators SalA and SyrF, Which Are Required for Syringomycin and Syringopeptin Production by Pseudomonas syringae pv. Syringae
… Polyclonal antiserum with antibodies recognizing SyrF-MBP was commercially produced by immunization of rabbits with purified SyrF protein (Pacific Immunology Corp., CA). Western blots were performed as described above. …
Nian Wang, Shi-En Lu, Angela R. Records, and Dennis C. Gross*
Journal of Bacteriology, May 2006, p. 3290-3298, Vol. 188, No. 9
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The C-Terminal Domain of MinC Inhibits Assembly of the Z Ring in Escherichia coli
… performed with blots containing equal amounts of cell protein per lane, as described previously (1). Purified MinD protein from strain BL21(DE3) carrying pWM1682 (pET28a-his-minD) was used to raise rabbit anti-MinD polyclonal antiserum (Pacific Immunology, Ramona, CA). …
Daisuke Shiomi and William Margolin *
Journal of Bacteriology, January 2007, p. 236-243, Vol. 189, No. 1
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Inactivation of Streptococcus gordonii SspAB Alters Expression of Multiple Adhesin Genes
… manufacturer. The membrane was then incubated with rabbit antiserum against paraformaldehyde-fixed cells of S. gordonii DL1 (Pacific Immunology Corp., Ramona, CA) and visualized as described for Western blotting. Statistics …
Yongshu Zhang,1 Yu Lei,1 Angela Nobbs,1 Ali Khammanivong,1 and Mark C. Herzberg1,2*
Infection and Immunity, June 2005, p. 3351-3357, Vol. 73, No. 6
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Fusobacterium nucleatum Transports Noninvasive Streptococcus cristatus into Human Epithelial Cells
… Production of Anti-MRSA PK Antibody Polyclonal PK antibody was produced by immunization of rabbit with purified recombinant His-tagged MRSA PK WT according to standard protocols (Pacific Immunology Corp, CA). …
Andrew M. Edwards, Tracy J. Grossman, and Joel D. Rudney*
Infection and Immunity, January 2006, p. 654-662, Vol. 74, No. 1
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