Cultivation and Serological Characterization of a Human Theiler’s-Like Cardiovirus Associated with Diarrheal Disease
… gels (Invitrogen, Carlsbad, CA) and Coomassie staining. Two milligrams of purified His 6 -VP1 was used for polyclonal antibody generation in rabbits (Pacific Immunology, Ramona, CA). VP1 ELISA.Maxisorp 96-well microtiter …
C. Y. Chiu,1,2* A. L. Greninger,4 E. C. Chen,1 T. D. Haggerty,6 J. Parsonnet,6E. Delwart,1,7 J. L. DeRisi,4,5 and D. Ganem2,3,5
JOURNAL OF VIROLOGY, May 2010, p. 4407–4414 Vol. 84, No. 9 0022-538X/10 doi:10.1128/JVI.02536-09
An Antiretroviral/Zinc Combination Gel Provides 24 Hours of Complete Protection against Vaginal SHIV Infection in Macaques
… MIV-150 [38] was synthesized by catalyzed exchange with tritium gas and purified by preparative HPLC (American Radiolabeled Chemicals, Inc., St. Louis, MO) and a rabbit polyclonal Ab against MIV-150 was custom prepared by Pacific Immunology Corp. (Ramona, CA). …
Jessica Kenney1#, Meropi Aravantinou1#, Rachel Singer1, Mayla Hsu1, Aixa Rodriguez1, Larisa Kizima1, Ciby J. Abraham1, Radhika Menon1, Samantha Seidor1, Anne Chudolij1, Agegnehu Gettie2, James Blanchard3, Jeffrey D. Lifson4, Michael Piatak Jr.4, Jose A. Fernández-Romero1, Thomas M. Zydowsky1, Melissa Robbiani1*
PLoS ONE 6(1): e15835. doi:10.1371/journal.pone.00158351/5/2011
A plant-specific calreticulin is a key retention factor for a defective brassinosteroid receptor in the endoplasmic reticulum
… based affinity chromatography (Amersham Biosciences). The eluent was separated by SDS/PAGE and the GST-CRT3C41 band was excised and sent to Pacific Immunology for antiserum production. The anti-CRT3 specific …
Hua Jin1, Zhi Hong, Wei Su, and Jianming Li2; Communicated by Joanne Chory, The Salk Institute for Biological Studies, La Jolla, CA, June 4, 2009 (received for review May 11, 2009)
Published online before print July 13, 2009, doi: 10.1073/pnas.0906144106 PNAS July 13, 2009
Mapping the Protein Interaction Network in Methicillin-Resistant Staphylococcus aureus
… the results of the systematic identification of protein−protein interactions for the hospital-acquired strain MRSA-252. Using a high-throughput pull-down strategy combined with quantitative proteomics …
Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J. Foster, Raymond H. See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R. McMaster, Robert C. Brunham, B. Brett Finlay, Neil E. Reiner
Journal of Proteome Research Article ASAP
Avian bornavirus is present in many tissues of psittacine birds with histopathologic evidence of proventricular dilatation disease
… d. SuperdexTM 200, GE Healthcare Technologies, Piscataway, NJ.; e. Amicon Ultra-15, Millipore, Billerica, MA.; f. Pacific Immunology, Ramona, CA…
Raj Raghav, Michael Taylor, Josepha DeLay, Davor Ojkic, David L. Pearl, Amy L. Kistler, Joseph L. DeRisi, Donald Ganem and Dale A. Smith1
Journal of Veterinary Diagnostic Investigation Vol. 22 Issue 4, 495-508 (2010)