Methods and compositions for detecting thyroglobulin in a biological sample
… conjugation reactions containing the three human thyroglobulin peptides SEQ ID NOS:2, 3, and 5 conjugated with KLH as described above were pooled and used to immunize two New Zealand White rabbits using the standard protocol (Pacific Immunology, Ramona, Calif.). The peptide-conjugate pool containing all three peptides …
Hoofnagle, Andrew N. (Seattle, WA, US) Wener, Mark (Seattle, WA, US)
US Patent Application Application Number: 12/139382 10/5/2010
Visit Citation Link
Immunoaffinity Purification of the Class V Chitin Synthase of Wangiella (Exophiala) dermatitidis
… purification were performed as follows: the WdMyo12p recombinant protein (unpublished), which is the HIS-tagged version of a conserved region in the MMD of WdChs5p expressed in E. coli strain BL21(DE3)pLys, was used for the immunization of rabbits (Pacific Immunology). …
Dariusz Abramczyk; Paul J. Szaniszlo
Preparative Biochemistry and Biotechnology, 1532-2297, Volume 39, Issue 3, 2009, Pages 277 – 288
Visit Citation Link
Functional Analysis, Overexpression, and Kinetic Characterization of Pyruvate Kinase from Methicillin-Resistant Staphylococcus aureus
… Production of Anti-MRSA PK Antibody Polyclonal PK antibody was produced by immunization of rabbit with purified recombinant His-tagged MRSA PK WT according to standard protocols (Pacific Immunology Corp, CA). …
Roya Zoraghi‡, Raymond H. See‡ , Huansheng Gong‡, Tian Lian‡, Rick Swayze‡, B. Brett Finlay§ , Robert C. Brunham‡ , William R. McMaster‡ and Neil E. Reiner*
Biochemistry, 2010, 49 (35), pp 7733–7747 DOI: 10.1021/bi100780t
Visit Citation Link
Experimental induction of proventricular dilatation disease in cockatiels (Nymphicus hollandicus) inoculated with brain homogenates containing avian bornavirus 4
… Fractions containing purified ABVN-His6 were combined and concentrated using a 15 kDa Amicon Ultra. 2.5 mg of this purified ABVN-His6 was used for polyclonal antibody generation in rabbits (Pacific Immunology; Ramona, CA). …
Ady Y Gancz1*†, Amy L Kistler2,3†, Alexander L Greninger2,3, Yigal Farnoushi4, Sara Mechani4, Shmuel Perl5, Asaf Berkowitz6, Noa Perez6, Susan Clubb7, Joseph L DeRisi2,3, Don Ganem2,3 and Avishai Lublin4
Virology Journal 2009, 6:100 doi:10.1186/1743-422X-6-100 Published:9 July 2009
Visit Citation Link
Execution of the Circadian Negative Feedback Loop in Neurospora Requires the ATP-Dependent Chromatin-Remodeling Enzyme CLOCKSWITCH
… In the Neurospora circadian system, the transcription factors White Collar-1 (WC-1) and White Collar-2 (WC-2) activate expression of frq, whose gene product inhibits its own expression. The WC proteins are thought to form an obligate …
William J. Belden,1 Jennifer J. Loros,1 and Jay C. Dunlap1,*
Molecular Cell 25, 587–600, February 23, 2007
Visit Citation Link