Formation and release of arrestin domain-containing protein 1-mediated microvesicles (ARMMs) at plasma membrane by recruitment of TSG101 protein
… endosomal sorting complex required for transport (ESCRT) machinery used forMVBformation to mediate the egress of viral particles from host cells. Here we report …
Joseph F. Nabhana, Ruoxi Hua, Raymond S. Oha, Stanley N. Cohenb,1, and Quan Lu
PNAS March 13, 2012 vol. 109 no. 11 4146-4151
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Deconstructing honeybee vitellogenin: novel 40 kDa fragment assigned to its N terminus
… After 3×15 min washes with PBS-T, sections were incubated overnight at 4°C with a polyclonal (rabbit) anti-vitellogenin antibody at 1:500 (raised against 180 kDa honeybee vitellogenin; Pacific Immunology, Ramona, CA, USA); specificity had been tested and confirmed …
Heli Havukainen, Øyvind Halskau, Lars Skjaerven, Bente Smedal1 and Gro V. Amdam
doi: 10.1242/jeb.048314 February 15, 2011 J Exp Biol 214, 582-592.
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Rot and SaeRS cooperate to activate expression of the staphylococcal superantigen-like exoproteins
… Generation of anti-Rot polyclonal antibody.The rot gene was amplified, cloned, expressed, and purified as described above. The Rot polyclonal antibody was generated by Pacific Immunology by immunizing rabbits with the purified protein. …
Meredith A. Benson, Sarit Lilo, Tyler Nygaard, Jovanka M. Voyich and Victor J. Torres
Published ahead of print 8 June 2012, doi: 10.1128/JB.00706-12 JB.00706-12
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Characterization of acyl carrier protein and LytB in Babesia bovis apicoplast.
… Antibody production of rmACP68-148 was 19 subsequently generated (Pacific Immunology Corp., CA), affinity-purified using 20 HiTrapâ„¢ Protein A HP Columns (GE Healthcare) and validated by western blot 21 analysis using B. bovis infected erythrocytes, uninfected …
Marina C. Caballero,a Monica J. Pedroni,b Guy H. Palmer,a Carlos E. Suarez,ab Christine Davitt,c and Audrey O.T. Laua
Mol Biochem Parasitol. 2012 February; 181(2): 125–133. doi: 10.1016/j.molbiopara.2011.10.009
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The complex that inserts lipopolysaccharide into the bacterial outer membrane forms a two-protein plug-and-barrel
… This glycolipid is found exclusively in the outer leaflet of the asymmetric outer membrane (OM), where it forms a barrier to the entry of toxic …
Elizaveta Freinkman, Shu-Sin Chng, and Daniel Kahne
PNAS January 21, 2011 10.1073/pnas.1015617108
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