Distinct immunoregulatory properties of macrophage migration inhibitory factors encoded by Eimeria parasites and their chicken host
… Immobilon-P, Millipore, Billerica, MA), and the membranes were treated with SuperBlock buffer (Fisher, Pittsburgh, PA) at 4°C for 16–24 h and incubated with rabbit antisera commercially prepared against recombinant E.MIF or C.MIF proteins (Pacific Immunology, Ramona, CA …
SI Jang, HS Lillehoj, SH Lee, DK Kim, M Pagés… –
Vaccine, 2011 – Elsevier
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The unfolded protein response is necessary but not sufficient to compensate for defects in disulfide isomerization
… 14). Western blotting was performed as described previously (8), using purified Pdi1p or PDIa′ as standards against customer-made yeast PDIa′ antiserum (Pacific Immunology Corp.) and anti-PGK (Invitrogen) as a control. …
JH Kim, Y Zhao, X Pan, X He, HF Gilbert –
Journal of Biological Chemistry, 2009 – ASBMB
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SREBP-Dependent Triazole Susceptibility in Aspergillus fumigatus Is Mediated through Direct Transcriptional Regulation of erg11A (cyp51A)
… according to the manufacturer’s instructions. (Bio-Rad). An affinity-purified rabbit polyclonal antibody was generated utilizing this recombinant protein by Pacific Immunology using standard protocols. The erg11A probe was amplified …
SJ Blosser, RA Cramer –
Antimicrobial Agents and Chemotherapy, 2012 – Am Soc Microbiol
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Three proteins of the U7-specific Sm ring function as the molecular ruler to determine the site of 3′-end processing in mammalian histone pre-mRNA
… Immunoprecipitation experiments under native or denaturing conditions were carried out as described previously (14). Antibodies.Anti-mouse 3′hExo and anti-human Lsm11 antibodies were generated in rabbits by Pacific Immunology (Ramona, CA). …
X Yang, MP Torres, WF Marzluff… –
Molecular and cellular …, 2009 – Am Soc Microbiol
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Use of immunomagnetic separation for the detection of Desulfovibrio vulgaris from environmental samples
… flagellar proteins. The resulting volume was preserved in PBS with 0.02 % thimerosal (Sigma, St. Louis, MO). The preparation was then shipped to Pacific Immunology Corporation (Ramona, CA) for processing. The purified …
R Chakraborty, TC Hazen, DC Joyner, K Küsel… –
Journal of …, 2011 – Elsevier
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