A Conserved Threonine Residue in the Juxtamembrane Domain of the XA21 Pattern Recognition Receptor Is Critical for Kinase Autophosphorylation and XA21-mediated Immunity
… SDS-PAGE (10% gel) was used to separate the proteins. Western blotting was performed as described previously (29) using anti-LexA (Clontech), anti-XB24 (Pacific Immunology), or anti-XB15 (Pacific Immunology) antibodies. Immunoprecipitation of Rice Proteins. …
1.Xuewei Chen, 2.Mawsheng Chern, 3.Patrick E. Canlas, 4. Caiying Jiang1, 5.Deling Ruan, 6.Peijian Cao and 7.Pamela C. Ronald2
The Journal of Biological Chemistry, 285, 10454-10463. 1/29/2010
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Tyrosine Phosphorylation of the Nuclear Receptor Coactivator AIB1/SRC-3 Is Enhanced by Abl Kinase and Is Required for Its Activity in Cancer Cells
… The antibodies used for IP were 4G10 phosphorylated tyrosine antibody (Ab) agarose conjugate (Upstate Biotech, Inc.), AIB1 monoclonal antibody (MAb) (BD Transduction Laboratories), phospho-Y1357 AIB1 polyclonal Ab (Pacific Immunology Co.), FLAG M2 affinity gel (Sigma …
Annabell S. Oh,1 John T. Lahusen,1 Christopher D. Chien,1 Mark P. Fereshteh,2 Xiaolong Zhang,3 Sivanesan Dakshanamurthy,1 Jianming Xu,4 Benjamin L. Kagan,1 Anton Wellstein,1,2 and Anna T. Riegel1,2*
Molecular and Cellular Biology, November 2008, p. 6580-6593, Vol. 28, No. 21
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The NIMA-family kinase Nek3 regulates microtubule acetylation in neurons
… To produce antibodies specific for Nek3 phosphorylated at Thr475, rabbits were immunized with peptides corresponding to Nek3 residues 466-481 [Cys-FEPRLDEEDT(-P)DFEEDN, where T(-P) represents phosphothreonine] at Pacific Immunology. …
Jufang Chang1, Robert H. Baloh2,3 and Jeffrey Milbrandt1,3,*
Journal of Cell Science 122, 2274-2282 (2009)
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ARID3B Induces Tumor Necrosis Factor Alpha Mediated Apoptosis While a Novel ARID3B Splice Form Does Not Induce Cell Death
… recognizing amino acids 510–560 only present in ARID3B FL). We also generated an antibody to the unique epitope found only in ARID3B Sh (ECASTHHSNSGNTDRVPTVC) (Pacific Immunology, Ramona, CA) …
Stancy Joseph1, Victoria E. Deneke4, Karen D. Cowden Dahl
PLoS ONE 7(7): e42159. doi:10.1371/journal.pone.0042159
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The Wilms’ Tumor Suppressor WT1 Inhibits Malignant Progression of Neoplastigenic Mammary Epithelial Cells
… Charlottesville, VA, USA) and affinity-purified rabbit polyclonal anti-ER-α36 antibody generated as a custom service from Pacific Immunology Corp. Ramona, CA, USA (15). The membranes were then incubated …
Lei Wang and Zhao-Yi Wang
Anticancer Res. 2008; 28(4B): 2155–2160.
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