Role in Diuresis of a Calcitonin Receptor (GPRCAL1) Expressed in a Distal-Proximal Gradient in Renal Organs of the Mosquito Aedes aegypti (L.)
… The membrane was incubated overnight in blocking buffer with 1:500 diluted rabbit anti-AaegGPRCAL1 (C-RGYAGTPEDTIE: second extracellular loop) affinity purified antibodies (0.5 mg/ml) (Pacific Immunology, Ramona, CA, USA) on a shaker overnight at 4°C. After washed …
H Kwon, HL Lu, MT Longnecker, PV Pietrantonio –
PLOS ONE, 2012
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Identification of DES1 as a vitamin A isomerase in Müller glial cells of the retina
… Equilibrium isomerization of retinol is a new activity now attributable to DES1. 11-cis-retinol synthesized by DES1 in Müller cells of the retina can be converted to the visual chromophore for regenerating opsin pigment in cone photoreceptors…
JJ Kaylor, Q Yuan, J Cook, S Sarfare, J Makshanoff… –
Nature Chemical Biology, 2012
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RivR is a negative regulator of virulence factor expression in the group A Streptococcus
… 186 187 Western blot analysis. Protein samples were separated on 12% Tris-HCl gels. An 188 affinity purified rabbit-anti-GRAB antibody was generated by Pacific Immunology Corp 189 from rabbits that were co-immunized with two peptide fragments of GRAB (Figure S1), 190 …
J Treviño, Z Liu, TN Cao, E Ramirez-Peña, P Sumby –
Infection and Immunity, 2012
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Transgenic zebrafish model of the C43G human insulin gene mutation
… Louis, MO, USA; 1:200), monoclonal mouse anti-KDEL (Abcam, Cambridge, MA, USA; 1:200), monoclonal rabbit anti-BiP (Cell Signaling, Danvers, MA, USA; 1:200) and monospecific rabbit anti-zebrafish C-peptide (Pacific Immunology, Ramona, CA, USA; 1:500). …
SC Eames, MD Kinkel, S Rajan, VE Prince, LH Philipson
Journal of Diabetes Investigation 2012
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Regulation of lipid synthesis by liver X receptor α and sterol regulatory element-binding protein 1 in mammary epithelial cells
… Western blot analysis of total ACC1 (rabbit anti-human ACC polyclonal antibody, 3662; Cell Signaling Technology Inc., Danvers, MA), SCD1 (custom synthesized rabbit anti-bovine antibody; Pacific Immunology Corp., Ramona, CA), FAS (rabbit anti-human FAS monoclonal …
C Oppi-Williams, JK Suagee, BA Corl
Journal of Dairy Science, 2012
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