The type II interleukin-1 receptor (IL-1RII) of the bony fish gilthead seabream Sparus aurata is strongly induced after infection and tightly regulated at transcriptional and post-transcriptional levels
… Blots were probed with specific antibodies to V5, Xpress (Invitrogen) or the C-terminus of sbIL-1β (Pacific Immunology), and developed with enhanced chemiluminescence (ECL) reagents (Amersham Biosciences) according to the manufacturer’s protocol. 2.8. …
Gloria López-Castejóna, M. Pilar Sepulcrea, Francisco J. Rocaa, Barbara Castellanab, Josep V. Planasb, José Meseguera and Victoriano Mulero
Molecular Immunology, Volume 44, Issue 10, April 2007, Pages 2772-2780
Non-AUG Translation Initiation of a Fungal RING Finger Repressor Involved in Photocarotenogenesi
… NY). Antibodies and Western Blotting—Rabbit monospecific antibodies were generated against the 18-amino acid CrgA peptide CLARRKRSEGEPPFFEYG, and purified by affinity chromatography (Pacific Immunology Corp.). …
Laura Murcia-Flores1, Juan M. Lorca-Pascual2, Victoriano Garre, Santiago Torres-Martínez, and Rosa M. Ruiz-Vázquez3
J. Biol. Chem., Vol. 282, Issue 21, 15394-15403, May 25, 2007
NOVEL MECHANISM OF ACTIVATION OF NADPH OXIDASE 5(NOX5): CALCIUM SENSITIZATION VIA PHOSPHORYLATION
… NY). Antibodies and Western Blotting—Rabbit monospecific antibodies were generated against the 18-amino acid CrgA peptide CLARRKRSEGEPPFFEYG, and purified by affinity chromatography (Pacific Immunology Corp.). …
Davin Jagnandan1, Jarrod E. Church1, Botond Banfi2, Dennis J. Stuehr3, Mario B. Marrero1, David J.R. Fulton
J. Biol. Chem., Vol. 282, Issue 9, 6494-6507, March 2, 2007
Determining the subcellular localization of a novel arginine kinase in Caenorhabditis elegans
… predicted an antigenic sequence unique to only this AK isozyme. Rabbit anti-AK polyclonal antibodies (Pacific Immunology) were generated and exhibited (by Western Blot) specific binding to the intended AK isozyme antigen. Future …
Baldassare D. Pipitone and Mark J. Snider
The FASEB Journal. 2006;20:A900
Upregulation of sterol C14-demethylase expression in Trypanosoma cruzi treated with sterol biosynthesis inhibitors
… 2.6. Peptides and antibodies. Synthetic peptides and monospecific antibodies were made as a service by Pacific Immunology (Ramona, CA). Detailed information about their methods can be obtained at Pacific Immunology (website: http://www.pacificimmunology.com/). …
Eleanor G. Hankins, John R. Gillespie, Kendra Aikenhead and Frederick S. Buckner*
Molecular and Biochemical Parasitology / Volume 144, Issue 1, November 2005, Pages 68-75