Cholesterol efflux in megakaryocyte progenitors suppresses platelet production and thrombocytosis
…The rabbit antibody to ABCG4 was custom made by Pacific Immunology (CA, USA) against a synthetic ABCG4 peptide (KKVENHITEAQRFSHLPKR). Monospecific anti-peptide antibodies werepurified using a peptide-affinity column. The specificity of the antibody forABCG4 protein was assessed by immunofluorescence microscopy, which showed specific immunofluorescence signals in HEK293 cells expressing ABCG4 but notHEK293 cells transfected with mock vectors…
Andrew J Murphy, Nora Bijl, Laurent Yvan-Charvet, Carrie B Welch, Neha Bhagwat, Adili Reheman, Yiming Wang, James A Shaw, Ross L Levine, Heyu Ni, Alan R Tall & Nan Wang
Nature Medicine 19, 586–594 (2013) doi:10.1038/nm.3150
Visit Citation Link
A detergent-free strategy for the reconstitution of active enzyme complexes from native biological membranes into nanoscale discs
…For immunodetection of mitochondrial proteins, samples were resuspended in SIB and solubilized with DDM or SMA as indicated. Following transfer onto nitrocellulose membranes, Western blotting was performed with primary antibodies against the Sdh3 subunit of respiratory Complex II (Pacific Immunology) or against the Tim50 subunit of the TIM23 Complex (a gift from Dr. Dejana Mokranjac) and Amersham ECL Plex Cy5-conjugated secondary antibodies.
Ashley R Long1, Catherine C O’Brien1, Ketan Malhotra1, Christine T Schwall1, Arlene D Albert1, Anthony Watts2 and Nathan N Alder
BMC Biotechnology 2013, 13:41 doi:10.1186/1472-6750-13-41
Visit Citation Link
Calcitonin receptor 1 (AedaeGPCRCAL1) hindgut expression and direct role in myotropic action in females of the mosquito Aedes aegypti (L.)
…The blot was incubated with rabbit anti-AedaeGPCRCAL1 (antigen sequence: C-RGYAGTPEDTIE corresponding to the receptor second extracellular loop) affinity purified antibodies (1:500 dilution; 1 mg/ml final concentration) (Pacific Immunology, Ramona, CA, USA) on a shaker at 4C overnight…
Hyeogsun Kwon, Patricia V. Pietrantonio
Insect Biochemistry and Molecular Biology Volume 43, Issue 7, July 2013, Pages 588–593
Visit Citation Link
EscE and EscG are co-chaperones for the type III needle protein EscF of enteropathogenic E. coli
…Type III secretion systems (T3SS) are central virulence mechanisms used by a variety of Gram-negative bacteria to inject effector proteins into host cells. The needle polymer is an essential part of the T3SS that provides the effector proteins a continuous channel into the host cytoplasm…
Neta Sal-Man1, Dheva Setiaputra1, Roland Scholz1, Wanyin Deng1, Angel C.Y. Yu2,4, Natalie C.J. Strynadka2,4 and B. Brett Finlay1,2,3,*
Journal of Bacteriology March 2013, doi: 10.1128/JB.00118-13
Visit Citation Link
Identification of novel breast cancer‐associated transcripts by uniGene database mining and gene expression analysis in normal and malignant cells
… BUC11 Immunohistochemistry in Testis and Breast Cancer Tissues. A custom-made polyclonal monospecific rabbit anti-BUC11 antibody (Pacific Immunology, CA) was tested on testis (cancer and normal) tissue microarray purchased from US Biomax (TE241). …
Stéphanie A.-S. Laversin1,†, Vinaya M. Phatak1,†, Des G. Powe1,2, Geng Li1,3, Amanda K. Miles1, David C. Hughes4, Graham R. Ball1, Ian O. Ellis2, Angelos D. Gritzapis5, Ioannis Missitzis6, Stéphanie E. B. McArdle1, Robert C. Rees1
Genes Chromosom. Cancer. doi: 10.1002/gcc.22031
Visit Citation Link