The Nup107-160 complex and γ-TuRC regulate microtubule polymerization at kinetochores
… immunizing rabbits. Antibodies against human Nup160 (amino acids 994–1316) and human GCP2 (amino acids 286–486) were also raised similarly. Rabbit polyclonal antibodies were generated by Pacific Immunology. To affinity …
Ram Kumar Mishra1, Papia Chakraborty2, Alexei Arnaoutov1, Beatriz M.A. Fontoura2 & Mary Dasso
Nature Cell Biology 12, 164 – 169 (2010) 17 January 2010 | doi:10.1038/ncb2016
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The directionality of the nuclear transport of the influenza A genome is driven by selective exposure of nuclear localization sequences on nucleoprotein
… The affinity-purified rabbit polyclonal antibodies against the NLSs of NP (NLS1, 1 MASQGTKRSYEQM 13 and NLS2, 198 KRGINDRNFWRGENGRKTR 216 ) were produced by Pacific Immunology, and have been characterized previously [7,8]. The mouse monoclonal NP …
Winco WH Wu and Nelly Panté*
Virology Journal 2009, 6:68 doi:10.1186/1743-422X-6-68
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TANGO1 Facilitates Cargo Loading at Endoplasmic Reticulum Exit Sites
… TANGO1 polyclonal antibody was raised in rabbits by immunization with keyhole limpet hemocyanin-conjugated peptide (C-SRDEPPPASQSTSQD) corresponding to the C-terminal amino acids 1884–1898 of TANGO1. The antibody was affinity purified on peptide column (Pacific Immunology, USA). Collagen VII polyclonal antibody to NC2 domain was used for western blot as described previously …
Kota Saito,1 Mei Chen,3 Fred Bard,4 Shenghong Chen,5 Huilin Zhou,5 David Woodley,3 Roman Polischuk,6 Randy Schekman,7 and Vivek Malhotra1,2,
DOI 10.1016/j.cell.2008.12.025
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RhoBTB2 (DBC2) Is a Mitotic E2F1 Target Gene with a Novel Role in Apoptosis
… RhoBTB2 Antibody Production—Affinity-purified rabbit polyclonal antibody was … RhoBTB2 Antibody Production—Affinity-purified rabbit polyclonal antibody was generated toward a peptide corresponding to human RhoBTB2 amino acids 673-687 (KEEDHYQRARKEREK) by Pacific Immunology (Ramona, CA). …
Scott N. Freeman ‡ § ¶ , • Yihong Ma ‡ § and • W. Douglas Cress
The Journal of Biological Chemistry, 283, 2353-2362
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Rac2 in murine pulmonary microvascular endothelial cells (ECs)
… An 11 amino acid region in the C-terminus of murine Rac2 was used to generate a rabbit polyclonal anti-Rac2 antibody (Pacific Immunology). This antibody recognized Rac2 …
John Clifford Gomez and Claire M. Doerschuk
FASEB J. April 2010 24 (Meeting Abstract Supplement) 777.11 “
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