Functional redundancy of steroid C26-monooxygenase activity in Mycobacterium tuberculosis revealed by biochemical and genetic analyses
… Alabaster, AL). Polyclonal antibodies to purified M. tuberculosis CYP124A1, CYP125A1, and CYP142A1 were raised in rabbits (Pacific Immunology, Ramona, CA). Cloning, Expression, and Purification. The CYP124A1, CYP125A1 …
JB Johnston, H Ouellet, PRO de Montellano –
Journal of Biological …, 2010 – ASBMB
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EscI: a crucial component of the type III secretion system forms the inner rod structure in enteropathogenic Escherichia coli
… 11 (Covance), diluted 1:1000; rat anti-HA (Roche), diluted 1:1000; mouse anti-V5 (Invitrogen), diluted 1:5000; goat anti-HSV (Abcam), diluted 1:400; rabbit anti-EscI (raised against a peptide of EPEC EscI with the sequence SPEQVLIEEIKKRHLA; Pacific Immunology), diluted 1 …
SM Neta, D Wanyin –
Biochemical Journal, 2012 – biochemj.org
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Immunocytochemical Tools Reveal a New Research Field Between the Boundaries of Immunology and Reproductive Biology in Teleosts
… gilthead seabream Mcsfr (SLRVVRKEGEDYLLPC). The two last antibodies were produced by Pacific Immunology and characterized by Chaves-Pozo et al. (2008a) and Mulero et al. (2008), respectively. We have also used …
A García-Ayala, E Chaves-Pozo –
cdn.intechopen.com
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Expression and function of the bile acid receptor GpBAR1 (TGR5) in the murine enteric nervous system
… New Zealand White rabbits were immunized with a peptide corresponding to the C-terminus of human GpBAR1 with an N-terminal Cys residue for conjugation to keyhole limpet hemocyanin (Cys-P 310 GPSIAYHPSSQSSVDLDLN 330 ) (Pacific Immunology, San Diego, CA …
D. P. Poole1,3, C. Godfrey1,3, F. Cattaruzza1, G. S. Cottrell1, J. G. Kirkland1,3, J. C. Pelayo1, N. W. Bunnett1,2, C. U. Corvera1,
2010 – Wiley Online Library
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Identification and characterization of functional aquaporin water channel protein from alimentary tract of whitefly, Bemisia tabaci
… 2.6. Antibody production. A synthetic peptide (IMP-1) corresponding to the final 16 residues of BtAQP1 (C-ITFKAKKRSDESSYDF) was identified by Pacific Immunology (Ramona, CA USA) to be potentially immunogenic. IMP …
LG Mathew, EM Campbell, AJ Yool…
Insect biochemistry and …, 2011 – Elsevier
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