… cells. Viruses were activated by incubation with 5 to 10 μg trypsin per ml for 30 to 60 min prior to infection. Antibodies.NSP1 polyclonal antiserum was produced by Pacific Immunology Corporation (Ramona, CA). Peptides corresponding …
MM Arnold, JT Patton –
Journal of virology, 2011 – Am Soc Microbiol

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… in Coons buffer to block the non-specific reaction, they were rinsed in CBT and incubated overnight at 4 °C with a purified rabbit polyclonal antiserum raised against a synthetic peptide corresponding to the C-terminus of the gilthead seabream Il1b (Pacific Immunology), at the …
E Chaves-Pozo, S Liarte, L Fernández-Alacid… –
Molecular …, 2008 – Elsevier

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… accession No. P07435 for the full-length OBP protein; Pacific Immunology Corporation, San Diego, CA). The antibody was applied at a 1:500 dilution in phosphate bufferedsaline (PBS) for 30 minutes at room temperature. After …
GB Mitchell, ME Clark, R Lu… –
Veterinary Pathology …, 2011 – vet.sagepub.com

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… Transfected cells were analyzed approximately 24 h upon transfection. Antibodies. Rabbit anti-TULA antibody was generated by Pacific Immunology (Ramona, CA) using a synthetic peptide corresponding to amino acids 317–332 of the short form of TULA. …
V Bertelsen, K Breen, K Sandvig, E Stang… –
Experimental cell …, 2007 – Elsevier

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… date. Rhodaminated GnIH was custom-made (Pacific Immunology, CA) and consisted of (rhodamine B)SIKPFSNLPLRF-NH2; code: Bentley 3N05-2-PAC(GnIH- 12); MW: 1844.5 (theoretical), 1843.7 (detected); >90% purity. …
GE Bentley, JP Jensen, GJ Kaur, DW Wacker… –
Hormones and …, 2006 – Elsevier

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