Immunolocalization of β- and δ-giardin within the ventral disk in trophozoites of Giardia duodenalis using multiplexlaser scanning confocal microscopy

… The extracts were pelleted by centrifugation at 5,000×g for 30 min, and the supernatant subjected to NiNTA affinity chromatography to purify recombinant β-giardin protein. Preparation of antisera Polyclonal sera specific for β- giardin was prepared by Pacific Immunology, Inc. …
Dumitru Macarisin & Celia O’Brien & Ronald Fayer & Gary Bauchan & Mark Jenkins
Parasitol Res. 2012 Feb 3. 10.1007/s00436-012-2825-x

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