Cultivation and Serological Characterization of a Human Theiler’s-Like Cardiovirus Associated with Diarrheal Disease
… gels (Invitrogen, Carlsbad, CA) and Coomassie staining. Two milligrams of purified His 6 -VP1 was used for polyclonal antibody generation in rabbits (Pacific Immunology, Ramona, CA). VP1 ELISA.Maxisorp 96-well microtiter …
C. Y. Chiu,1,2* A. L. Greninger,4 E. C. Chen,1 T. D. Haggerty,6 J. Parsonnet,6E. Delwart,1,7 J. L. DeRisi,4,5 and D. Ganem2,3,5
JOURNAL OF VIROLOGY, May 2010, p. 4407–4414 Vol. 84, No. 9 0022-538X/10 doi:10.1128/JVI.02536-09